Diameter of the mammalian porin channel in open and "closed" states: direct measurement at the single channel level in planar lipid bilayer |
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Authors: | OV Krasilnikov CM Carneiro LN Yuldasheva AC Campos-de-Carvalho RA Nogueira |
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Affiliation: | Laboratory of Molecular Physiology, Institute of Physiology and Biophysics, Tashkent, Uzbekistan. kras@npd.ufpe.br |
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Abstract: | Porin isolated from bovine skeletal muscle was reconstitute in planar lipid bilayers under voltage clamp conditions. A set of non-electrolytes were used as molecular probes for determining the pore diameter. The maximal diameter of the open channel was estimated to be 3.02 +/- 0.26 nm. As observed for other porin channels, a large transmembrane potential drove the channel into a "closed" state. The channel transition to the low conductance (closed) state was followed by a decrease in the maximal diameter of the channel to 2.4 +/- 0.08 nm. |
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