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Electrochemical properties of self-assembled cytochrome c on gold substrate patterned with a photosensitive polyimide film
Affiliation:1. Department of Chemical Engineering, Sogang University, 1 Shinsoo-Dong, Mapo-Gu, Seoul 121-742, South Korea;2. Department of Chemistry, Yonsei University, Wonju, Kangwon-do 220-710, South Korea;1. Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany;2. Department of Oral Biotechnology, Center for Dental Medicine, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany;1. Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;2. Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;1. Septomics Research Center, Friedrich Schiller University Jena, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institute, Jena, Germany;2. Center for Sepsis Control and Care, University Hospital Jena, Jena, Germany;3. Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institute, Jena, Germany;4. Faculty of Biology and Pharmacy, Friedrich Schiller University Jena, Jena, Germany;5. German National Reference Center for Invasive Fungal Infections, Hans-Knoell-Institute, Jena, Germany;1. Fertility Center Cancún, Cancún, Mexico;2. Departamento de Ciencia Animal y de los Alimentos, Facultad de Veterinaria, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain;3. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 Mexico City, Mexico;4. Departamento de Reproducción Animal, INIA, 28040 Madrid, Spain
Abstract:Photosensitive polyimide having benzene and sulfonyloxyimide moieties was prepared and microarray pattern on gold substrate was obtained by a UV lithographic technique. The well-characterized monolayer of cytochrome c was immobilized on the patterned gold substrate by self-assembly process. The bioelectrochemical activity between cytochrome c molecular center and electrode interface for the self-assembled cytochrome c monolayer was investigated through the measurement of cyclic voltammetry. The integration and morphology of cytochrome c molecule through the measurement of AFM was also examined.
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