芹菜不同部位提取物对调节大鼠高血压与血脂代谢紊乱作用机制研究

本实验通过芹菜素与芹菜不同部位(根、茎、叶)提取物对高盐诱导高血压大鼠模型进行调节,结合血脂代谢情况分析其作用效果。将成模大鼠分为模型组、实验组(芹菜素,芹菜根、茎、叶提取物)、给药对照组,以健康大鼠作为正常组,实验各组按照剂量灌胃芹菜不同部位提取物与芹菜素,模型组与正常组给予直饮水,治疗4周后,测定大鼠血浆中血管紧张素Ⅰ(AngⅠ)、血管紧张素Ⅱ(AngⅡ)、醛固酮(ALD)、肾素活性(PRA)、甘油三脂(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)和胆固醇(CHOL)含量变化,计算心脏指数(CMI)与心肌增值系数(MPI),结合心脏病变情况观察(HE染色)对其治疗效果进行综合分析。结果表明,与模型组相比,芹菜不同部位提取物与芹菜素能有效降低血浆中PRA(P<0.05),降低AngⅡ含量(P<0.05),达到降低动脉血压的效果;能够改善大鼠血脂代谢异常的情况,有效升高血浆HDL-C水平,降低LDL-C、TG与CHOL含量,降低CWI、MPI,从而减轻大鼠因心脏肿胀而造成的大鼠心负担过重,减轻心肌细胞水肿,减少心肌纤维断裂,维持心肌细胞细胞核完整性。尤以芹菜叶食疗药用价值最为明显,芹菜各部位提取物种中非芹菜素特征图谱黄酮物质含量颇丰,其相关生物活性机制具有较高潜在研究价值。

Mechanism of Extracts from Different Parts of Celery on Regulating Hypertension and Dyslipidemia in Rats

In this experiment,the effects of different concentrations of apigenin and celery(root,stem,leaf)extracts on hyperlipidemia-induced hypertensive rat model combined with lipid metabolism were analyzed. The model rats were divided into model group,experimental group(apigenin,celery root,stem and leaf extract),administration control group,healthy rats were used as normal group,and experimental groups were given extracts from different parts of celery and apigenin according to the dose. The model group and the normal group were given direct drinking water. After 4 weeks of treatment,the changes of angiotensinI(AngI),angiotensinII(Ang II),aldosterone(ALD),renin activity(PRA),triglyceride(TG),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C)and cholesterol(CHOL)content were measured in rat plasma. cardiac index(CMI)and myocardial value-added coefficient(MPI)were calculated,the therapeutic effect was comprehensively analyzed combined heart disease observation(HE staining). Results showed that,compared with the model group,extracts from different parts of celery could effectively reduce plasma PRA(P<0.05)and decrease AngII(P<0.05),and reduce arterial blood pressure. It could improve the abnormal blood lipid metabolism in rats and effectively increase the plasma HDL-C level,decrease the content of LDL-C,TG and CHOL,reduce CWI and MPI,so as to reduce the heart burden of rats caused by heart swelling,reduce myocardial cell edema and myocardial fiber breakage,and maintain the nuclear integrity of myocardial cells. Especially,the medicinal value of celery leaves was the most significant. The flavonoids in the celery extracts of various parts of celery were abundant,and the related biological activity mechanism had high potential research value.