复配菌对含蜡原油含蜡量及其黏度作用分析

微生物能改善含蜡原油的流动性，提高运输效率。将已培养的枯草芽孢杆菌6#和铜绿假单胞菌3#按2∶3的体积比复配培养，并优化其生长条件。采用DSC、偏光显微镜、HAKEE流变仪和界面张力仪对复配菌与含蜡原油的作用效果进行分析。经复配菌处理后，含蜡原油的析蜡点降低了2.23 ℃，析蜡高峰点降低了2.71 ℃，原油含蜡量（原油中蜡质量分数）降低51.64%；蜡晶结构发生了明显变化；37 ℃下测得原油表观黏度降低了64.72%；复配菌对液体石蜡的乳化指数为55.39%，液体石蜡培养基的表面张力从79.89 mN/m 降低至38.83 mN/m。与单菌相比，复配菌在除蜡降黏方面效果更为优异，应用前景更为广阔。

Effect of Compound Bacteria on Wax Content and Viscosity of Waxy Crude Oil

Microbes can improve the fluidity of waxy crude oil and increase the efficiency of transportation. The cultured Bacillus subtilis 6# and Pseudomonas aeruginosa 3# were mixed and cultured in a ratio of 2∶3, the growth conditions were optimized. DSC, polarizing microscope, HAKEE rheometer and interfacial tensiometer were used to analyze the effect of compound bacteria and waxy crude oil. The results showed that: after treatment with compound bacteria, the wax precipitation point of waxy crude oil decreased by 2.23 ℃, the peak point of wax precipitation decreased by 2.71 ℃, and the wax content of crude oil decreased by 51.64%; the wax crystal structure changed significantly; the apparent viscosity of crude oil decreased by 64.72% at 37 ℃. In addition, the emulsification index of the compound bacteria on liquid paraffin was 55.39%, and the surface tension of the liquid paraffin culture medium was reduced from 79.89 mN/m to 38.83 mN/m. Compared with single bacteria, compound bacteria are more effective in removing wax and reducing viscosity, and have wider application prospects.