一株假单胞菌的分离鉴定及其产絮凝剂絮凝特性的研究

目的:获得高效廉价的微生物絮凝剂。方法:利用常规筛选技术从宜州龙江河沿岸排污口污泥中分离筛选菌株,菌种鉴定是经形态观察、理化特性分析以及分子水平16S rDNA系统进化分析进行的;按照常规方法考察了絮凝剂在高岭土、活性炭及酵母悬浮液中的絮凝性能和影响絮凝性能的因素。结果:获得一株产絮凝剂的菌株L2019-2,该絮凝活性菌株鉴定为维罗纳假单胞菌(Pseudomonas veronii)。其产生的生物高分子絮凝剂在无机悬浮液和有机悬浮液中均具有絮凝活性。在高岭土悬浮液中,pH为5.0、温度为30 ℃、助凝剂为Fe³⁺时,絮凝剂的絮凝活性较高,絮凝率达98.30%。经化学组成分析,该絮凝剂为酸性糖蛋白。结论:L2019-2所产絮凝剂具有很好的絮凝性能,可为研究微生物絮凝剂提供良好的菌种资源,在污水处理中更具有很好的应用潜力。

Isolation,Identification and Flocculation Characteristics of a Pseudomonas Strain

Objective:In order to obtain high efficiency and low cost microbial flocculant.Methods:Using conventional screening techniques to isolate and screen strains from the sewage sludge along the Longjiang River in Yizhou. The identification of the strains was carried out through morphological observation,physical and chemical analysis and molecular level 16S rDNA systematic evolution analysis;according to conventional methods,the flocculant was investigated in kaolin. The flocculation performance in activated carbon and yeast suspension and the factors affecting flocculation performance.Results:L2019-2 was identified as Pseudomonas veronii. The flocculant produced by L2019-2 had flocculant activity in both inorganic and organic suspensions.The flocculant’s flocculant activity was influenced by cation,pH,temperature and other factors. When the pH was 5.0,the temperature was 30 ℃ and the coagulant aid was Fe3+,the flocculant had the optimum flocculating activity in kaolin suspension. After chemical composition analysis,the flocculant was acid glycoprotein.Conclusion:The flocculant produced by L2019-2 has good flocculating activity,which can provide good strain resources for the study of microbial flocculant,and has better application potential in sewage treatment.