| 铁皮石斛糖蛋白对皮肤炎症期的调控作用及机制 |
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| 引用本文： | 赵倩,戴天浥,洪文龙,周丽免,苏海冉,田洋,白忠彬.铁皮石斛糖蛋白对皮肤炎症期的调控作用及机制[J].食品工业科技,2020,41(21):304-310,316. |
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| 作者姓名： | 赵倩 戴天浥 洪文龙 周丽免 苏海冉 田洋 白忠彬 |
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| 作者单位： | 1. 云南农业大学食品科学技术学院, 云南昆明 650201;2. 云南农业大学国家辣木加工技术研发专业中心, 云南昆明 650201;3. 云南斛哥药业有限公司, 云南普洱 665000;4. 食药资源开发与利用教育部工程中心, 云南昆明 650201 |
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| 基金项目： | 云南省引进人才科研启动项目(A3007330)云南省中青年学术和技术带头人后备人才项目(2018HB040)。云南农业大学人才引进科研启动项目(A2002320) |
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| 摘 要： |
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| 关 键 词： | 铁皮石斛 糖蛋白 炎症因子 核转录因子(NF-κB) 伤口愈合 |
| 收稿时间： | 2019-12-10 |
Regulatory Effect of Dendrobium officinale Kinura et Migo Glycoprotein on Skin Inflammation and Its Mechanism |
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| ZHAO Qian,DAI Tian-yi,HONG Wen-long,ZHOU Li-mian,SU Hai-ran,TIAN Yang,BAI Zhong-bin.Regulatory Effect of Dendrobium officinale Kinura et Migo Glycoprotein on Skin Inflammation and Its Mechanism[J].Science and Technology of Food Industry,2020,41(21):304-310,316. |
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| Authors: | ZHAO Qian DAI Tian-yi HONG Wen-long ZHOU Li-mian SU Hai-ran TIAN Yang BAI Zhong-bin |
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| Affiliation: | 1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;2. National R & D Center for Moringa Processing Technology, Kunming 650201, China;3. Yunnan Huge Pharmaceutical Co., Ltd., Puer 665000, China;4. Food and Drug Resources Development and Utilization Engineering Center of the Ministry of Education, Kunming 650201, China |
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| Abstract: | The aim of this study was to investigate the regulation and mechanism of Dendrobium officinale Kinura et Migo glycoprotein(DOKMG)on skin inflammation.In this paper,macrophage cell line RAW264.7 was targeted and stimulated by DOKMG in different time and concentration,the mRNA expression levels of inflammatory factor IL-1β,IL-6,TNF-α and inflammation-related COX-2 and iNOS were detected by RT-PCR. The expression of inflammatory factors in supernatant of macrophages was detected by ELISA. Western blot was used to analyze the effect of DOKMG on the expression of inflammatory factor protein and NF-κB signaling pathway. The results showed that DOKMG significantly promoted the expression of IL-1β,IL-6,TNF-α genes after 24 h(P<0.001).When the concentration reached 100 μg/mL,the improvement was the most obvious. Compared with the control group,IL-1β increased by 10.09 times,IL-6 increased by 20.45 times,and TNF-α increased by 0.95 times. When the concentration was 100 μg/mL,the secretion of TNF-α、IL-1β increased from 0.52 μg/mL and 118.52 pg/mL to 1.99 μg/mL and 272.81 pg/mL,the expression of inflammatory cytokines and phosphorylation of NF-κB were significantly promoted(P<0.05).These results indicate that DOKM can significantly promote macrophage inflammatory response to promote wound healing,and its mechanism may be related to the activation of NF-κB signaling pathway. |
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