利用AuNCs@GSH-CAP体系荧光增强检测BSA的研究

蛋白质的研究是当下的热点，目前已经涉及到了生物、医学、食品等各个领域，而纳米技术的发展极大程度上促进了对蛋白质的研究。简便、高灵敏、高选择性的检测方法是实现重大疾病早期诊断和治疗的关键，也是目前市场潜力巨大的方向。但是许多检测方法的荧光探针合成较为复杂以及成本高，且需要使用有毒性的有机试剂。本文中，我们采用氯霉素（CAP）荧光猝灭的金纳米簇（AuNCs@GSH）体系作为探针，通过体系荧光增强效应来检测牛血清白蛋白（BSA）。BSA可以增强AuNCs@GSH-CAP体系荧光强度，在一定浓度范围内体系荧光增强与BSA的浓度呈正相关。该方法具有较好的选择性和抗干扰性，且荧光效果明显，实现了在10~160μmol/L内具有良好的线性关系，其检测限为0.1μmol/L，对于蛋白质的快速检测有良好的发展前景。

BSA Detection Using AuNCs@GSH-CAP Fluorescence Enhancement System

Protein research is a hot spot at present, and has been involved in biology, medicine, food and other fields, and the development of nanotechnology has greatly promoted the research on protein. Simple, highly sensitive and highly selective detection is the key to the early diagnosis and treatment of major diseases, and it is also the direction with great market potential. However, the synthesis of fluorescent probes for many detection methods is complex and costly, and requires the use of toxic organic reagents. In this study, we detected bovine serum albumin (BSA) using a gold nanocluster (AuNCs@GSH) system with fluorescence quenching by chloramphenicol (CAP) as a probe. BSA could enhance the fluorescence intensity of AUNCs-GSH-CAP system, which was positively correlated with the concentration of BSA within a certain concentration range. This method has good selectivity and anti-interference, and the fluorescence effect is obvious. It has achieved a good linear relationship within 10-160 mol/L, and its detection limit is 0.1 mol/L, which has a good prospect for the rapid detection of proteins.