壳聚糖复方对高脂饲料致肥胖大鼠减肥作用的研究

为研究壳聚糖复方(壳聚糖、白芸豆提取物、葡萄籽提取物和富硒酵母)的减肥作用,除空白组外,其余大鼠喂养高脂饲料2周建立肥胖大鼠模型,建模成功后再随机分为模型组、奥利司他组(48 mg/kg)、壳聚糖配方高(0.812 g/kg)、中(0.406 g/kg)、低(0.203 g/kg)剂量组,连续给药6周后检测各组大鼠体重、Lee's指数、肝体比、脂体比,血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、瘦素、脂联素、谷丙转氨酶(ALT)、谷草转氨酶(AST)及肝脏超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-Px)等指标。结果显示与模型组相比,壳聚糖复方高剂量能显著降低肥胖大鼠的终末体重、肝体比、血清TG、TC含量及AST、ALT活性(P<0.05),极显著降低Lee's指数、脂体比、血清LDL-C、瘦素及肝脏MDA含量(P<0.01),极显著提高血清脂联素含量及肝脏SOD活性(P<0.01),显著提高肝脏GSH-Px酶活力(P<0.05);壳聚糖复方中剂量能显著降低肥胖大鼠的终末体重、Lee's指数、肝体比、脂体比、TG、TC、瘦素、MDA含量及血清ALT、AST活力(P<0.05),极显著降低LDL-C含量(P<0.01),显著升高血清脂联素含量及肝脏SOD活性(P<0.05),表明壳聚糖复方具有一定的减肥作用,其作用机制可能与调节机体脂肪代谢相关激素水平,加快机体的脂质代谢,减少氧化损伤等有关。

Antiobesity Function of the Chitosan Compounds on Fatty Rats Established by High Fat Diet

To study the antiobesity function of the chitosan compounds (chitosan, white kidney bean extracts, grape seed extract and selenium-enriched yeast), except the blank control group rats, the rest rats were feed with high fat diet for 2 weeks to establish fatty animal models, and then the successful model of the rats were randomly divided into model group, orlistat group (48 mg/kg), chitosan compounds high (0.812 g/kg), middle (0.406 g/kg) and low (0.203 g/kg) dose groups. After 6 weeks of administration, the changes of weight, Lee's index, liver index, fat index, the contents of serum triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), leptin, adiponectin, liver malondialdehyde (MDA), and the activity of serum alanine aminotransferase (ALT), glutamic oxaloacetylase (AST), liver superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were detected. Compared with the rats in model group, the high dose of chitosan compounds could significantly lower the weight, liver index, the contents of serum TG and TC, the activity of AST and ALT (P<0.05), remarkably lower the Lee's index, fat index, the contents of the serum LDL-C, leptin, and the liver MDA (P<0.01), remarkably improve the contents of the serum adiponectin and the activity of liver SOD (P<0.01) and significantly improve the activity of liver GSH-Px (P<0.05). The middle dose of chitosan compounds (0.406 g/kg) could significantly lower the weight, Lee's index, liver index, fat index, the contents of the serum TG, TC, leptin and liver MDA, the activity of serum ALT and AST (P<0.05), and remarkably lower the contents of LDL-C (P<0.01), and significantly improve the contents of adiponectin and the activity of liver SOD (P<0.05). Chitosan compounds had the antiobesity function, and its mechanism would be related to regulating the level of hormone related to fat metabolism, accelerating lipid metabolism and reducing oxidative damage.