复方鹿角灵芝茶的制备及其免疫调节功能研究

为了探讨鹿角灵芝、富硒茶和紫苏提取物制成复方粉剂的免疫调节作用,分别提取其有效成分,采用单因素和正交试验优化复方鹿角灵芝茶颗粒的制备工艺;选用正常BALB/c小鼠为研究对象,测定复方鹿角灵芝茶对小鼠细胞、体液免疫调节功能以及巨噬细胞吞噬功能的影响。结果表明:复方鹿角灵芝茶颗粒最佳配方为主药为鹿角灵芝粗多糖0.38 g,富硒粗茶多酚1.24 g和紫苏粗多酚33 mg,主药:甘露醇为1:3,乙醇浓度为85%,聚维酮K30含量为4%。复方颗粒的大小均匀,色泽一致,符合2015版《中华人民共和国药典》的各项指标;复方鹿角灵芝茶能够提高小鼠细胞因子IL-10、IL-2和IFN-γ的分泌量及体液免疫功能,增强巨噬细胞对鸡红细胞的吞噬能力,为复方鹿角灵芝茶的制备及工业化生产提供理论依据。

Preparation and Immunoregulatory Function of Compound Antler Ganoderma lucidum Tea

In order to explore the immunomodulatory effects of extracts from antler Ganoderma lucidum, selenium-enriched tea and Perilla frutescens, extracted respectively their active ingredients and the preparation process of compound antler Ganoderma lucidum tea granules was optimized by single factor and orthogonal test. Then the normal BALB/c mice were selected as the research object to determine the effects of compound antler Ganoderma lucidum tea in the mouse cell, humoral immune regulation and macrophage phagocytosis in mice. The results showed that the best formula of compound antler Ganoderma lucidum tea granules was that the main grug was 0.38 g of crude polysaccharide antlers Ganoderma lucidum, 1.24 g of selenium-rich crude tea polyphenols and 33 mg of Perilla crude polyphenols, and the main drug to mannitol was 1:3, the ethanol concentration was 85%, and the povidone K30 content was 4%. The compound granules were uniform in size and color, and conformed to the indexes of Pharmacopoeia of the People's Republic of China (2015). The compound antler Ganoderma lucidum tea could increase the secretion of cytokines IL-10, IL-2 and IFN-γ and humoral immune function of mice, and enhance the phagocytosis of chicken red blood cells by macrophages. It would provide a theoretical basis for the preparation and industrial production of compound antler Ganoderma lucidum tea.