芋螺毒素cr5a的电喷雾四极杆飞行

cr5a是从中国南海软体动物芋螺的毒液中分离出的一种新的芋螺毒素。为了验证已得到的氨基酸序列分析结果,利用纳升电喷雾四极杆飞行时间串联质谱对cr5a的碘代乙酰胺(IAM)衍生物进行了串联质谱从头测序分析,采用MassLynx 3.5系统进行数据采集与处理,通过MassSeq软件提取串联质谱所得的氨基酸序列。软件提取的序列CCKFQFLNFCCNE与N末端序列分析结果相符,使得cr5a的氨基酸序列得到进一步验证。

Analysis of New Conotoxin cr5a by nano Q-ESI-TOF-MS/MS

cr5a is a new conotoxin isolated from the venom of conus carateristicus,which is one kind of gastropod mollusks in South China Sea.In order to validate the sequence,the iodoacetamide derivative of cr5a was analyzed by nano quadrupole electrospray ionization time of flight tandem mass spectrometry(nano Q-ESI-TOF-MS/MS).Tandem mass spectral data were acquired and processed with MassLynx 3.5,and then amino acid sequences were deduced with MassSeq software according to the mass differences between y-or b-ion ladder series and confirmed by manual interpretation.The sequence of CCKFQFLNFCCNE is identical with the result of N-terminal Edman sequence analysis,and the result is validated further.