| 用GC-MS法测定血浆中~(15)N-甘氨酸 |
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| 引用本文： | 江骥,王世真,潘炯光,徐植灵,王光辉. 用GC-MS法测定血浆中~(15)N-甘氨酸[J]. 核电子学与探测技术, 1984, 0(1) |
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| 作者姓名： | 江骥 王世真 潘炯光 徐植灵 王光辉 |
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| 作者单位： | 中国医学科学院首都医院(江骥，王世真)，中医研究院中药研究所(潘炯光，徐植灵)，中医研究院中药研究所(王光辉) |
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| 摘 要： | 本文对用GC-MS法测定血浆中~(15)N-甘氨酸进行了初步探讨。实验主要内容包括:(1)两种氨基酸衍生物(N-三氟乙酰甘氨酸正丁酯和N-七氟丁酰甘氨酸异丁酯)的制备;(2)混合氨基酸衍生物的分离;(3)同位素丰度的GC-MS测定。方法较为简便,灵敏、省时和精确。只需0.5ml全血即可满足一次样品的测定要求。本法对单纯甘氨酸样品测量的精确度为0.6％,混合氨基酸样品测定的实际误差在3％左右。
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| 关 键 词： | 甘氨酸 氨基酸 ~(15)N 同位素丰度 气相层析-质谱法 |
The Determination of ~(15)N-G1ycine in Plasma by Gas Chromatography-Mass Spectrometry |
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| Jiaug Ji Wang Shizhen Pan Jiongguang XuZhiling Wang Guanghui. The Determination of ~(15)N-G1ycine in Plasma by Gas Chromatography-Mass Spectrometry[J]. Nuclear Electronics & Detection Technology, 1984, 0(1) |
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| Authors: | Jiaug Ji Wang Shizhen Pan Jiongguang XuZhiling Wang Guanghui |
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| Abstract: | A method for the determination of 15N-glycine in plasma by GC-MS is dealt with. The procedure consists of: (1) Preparation of N-heptaf luorobutyl-glycine isobutyl ester and N-trifluoroacetyl-glycine n-butyl-ester; (2)Separation of mixed amino acid: (3)Detetrm-ination of isotopic abundance by GC-MS. This method is simple sensitve, accurate and timesaving.0.5ml of whole blood is sufficient for each determination.The accracy is 0.6%for glycine and 3% for mixed amino acid. |
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| Keywords: | Glycine Amino acid Nitrogen-15 Isotopic abundance Gas chroma-tography-mass spectrometry |
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