苎麻微生物脱胶特异菌株的筛选与鉴定

苎麻传统的化学脱胶办法存在环境污染以及对苎麻纤维具有较大的损伤等缺陷,而应用生物脱胶的办法则可以很好地克服化学脱胶所存在的弊端。通过从野外采集土样,采取多次富集、初筛复筛的手段得到1株对苎麻具有较强脱胶能力的菌株,命名为Dazu 5-1。利用该菌株对苎麻进行2 d发酵脱胶,平均减重率达到30.2%,与化学脱胶相比,经过Dazu 5-1菌株脱胶后的苎麻单纤维强力平均值提高76.93%;对Dazu 5-1菌株进行了形态学观察,生理生化指标测定,16S rDNA碱基序列测定和同源性分析,鉴定该菌株为志贺菌属。

Screening and identification of a bacterium strain for specific ramie degumming

In this paper, a bacterium strain with high-efficient ramie degumming , named Dazu5-1, was isolated from field soil by iterative enrichment,screening and rescreening methods. When the strains are used for 2d fermentation degumming of ramie, the average weight loss rate can reach 30.2%. Compared with the chemical degumming, Dazu5-1 strain degumming can make the average value of single fiber strength of ramie increase by 76.93%. Finally, the Dazu5-1 strain is identified as Shigella by morphological observation, physiological and biochemical indexes determination, 16SrDNA base sequencing and homology analysis.