| HPLC法测定陆英花和果实中齐墩果酸和熊果酸的含量 |
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| 引用本文： | 邹盛勤.HPLC法测定陆英花和果实中齐墩果酸和熊果酸的含量[J].生命科学仪器,2009,7(8):37-40. |
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| 作者姓名： | 邹盛勤 |
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| 作者单位： | 宜春学院化学与生物工程学院,江西省天然药物活性成分研究重点实验室,江西,宜春,336000 |
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| 基金项目： | 国家"863"计划重点资助项目 |
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| 摘 要： | 建立高效液相色谱法同时分离和测定陆英花和果实中齐墩果酸和熊果酸的含量。选用Kromasil C18色谱柱，流动相为甲醇：水：磷酸（90：10：0．2），检测波长210nm，流速0．8mL／min，柱温25℃。齐墩果酸进样量在n55—5．50μg，熊果酸进样量在1．02—10．20μg时呈现良好的线性关系，相关系数均为0．9999，齐墩果酸和熊果酸的平均回收率分别为99．3％（RSD为1．9％）和101．2％（RSD为2．3％）.本法具有准确、灵敏、数据可靠的优点，可用于陆英花和果实的齐墩果酸和熊果酸的分离与测定。
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| 关 键 词： | 高效液相色谱法 陆英 花 果实 齐墩果酸 熊果酸 含量测定 |
Determination of Ursolic Acid and Oleanolic Acid in Flowers and Fruits of Sambucus Chinensis Lind1. by HPLC |
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| Sheng-qin Zou.Determination of Ursolic Acid and Oleanolic Acid in Flowers and Fruits of Sambucus Chinensis Lind1. by HPLC[J].Life Science Instruments,2009,7(8):37-40. |
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| Authors: | Sheng-qin Zou |
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| Affiliation: | Sheng-qin Zou ( College of Chemistry and Bioengineering, Yichun University, Key laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun 336000,China ) |
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| Abstract: | A method for determination of oleanolic acid and ursolic acid simultaneously in flowers and fruits of Sambucus Chinensis Lindl was established. HPLC was carried out on a Kromasil C18 column using MeOH ： H20 ： H3PO4 （ 90 ： 10 ： 0.2, with detection at 210 nm, flow rate 0.8 ml/min and column temperature 25 ℃. An average recovery of 99.3 % was obtained with a RSD of 1.9 % in the range of 0.55-5.50 μg for oleanolic acid, and an average recovery of 101.2 % was obtained with a RSD of 2.3 % in the range of 1.02-10.20 μg for ursolic acid. There were both good linear correlations with the correlation coefficients of 0.9999.. The method is accurate, sensitive and the data is reliable, and it can be used for the isolation and determination of oleanohc acid and ursolic acid in flowers and fruits of Sambucus Chinensis Lindl. |
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| Keywords: | HPLC Sambucus ehinensis lindl flowers fruits ursolic acid oleanolic acid determination |
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