氨基酸螯合铜对育成蛋鸡免疫功能的影响

旨在观察不同水平的氨基酸螯合铜对育成蛋鸡免疫功能的影响及其机理探讨,以氨基酸螯合铜作为饲料添加的铜源,以216只9周龄的海兰褐蛋鸡为试验动物,随机分成6组(每组三个重复),10周龄时开始进入正式试验,试验期6周,分别饲喂添加氨基酸螯合铜(以铜量计)0(Ⅰ组)、6.5(Ⅱ组,对照组)、15(Ⅲ组)、30(Ⅳ组)、60(Ⅴ组)、125 mg/kg(Ⅵ组)相同的基础日粮.分别在鸡第12、14、16周龄时,称重、采血,并从每重复随机选取一只鸡,宰杀.采集血液样品和组织样品,进行淋巴细胞转化率、新城疫抗体水平、免疫器官指数的测定.结果表明:与对照组(第Ⅱ组)相比,第Ⅳ组显著提高蛋鸡胸腺指数(P<0.05),第Ⅲ组和第Ⅳ组可显著提高蛋鸡脾脏和法氏囊的免疫指数、淋巴细胞转化率和鸡新城疫抗体效价(P<0.05),说明添加15和30MG/kg的铜可增强育成蛋鸡的免疫功能.由此推测,15、30 mg/kg的铜为育成期蛋鸡最适宜的添加范围.

Copper- Amino Acid Chelate on Immune Function of Growing Layers

This work was conducted to study the effect of dietary copper- amino acid chelate (Cu - amino acid) on immune function in growing layers and to explore the mechanism. Two- hundred and sixteen 9 - week- age Hailan brown growing layers were used in a 6 - week trial to examine the effect of different levels of Cu- amino acid on immune function.The growing layers were divided randomly into 6 groups. Dietary treatments were 0 (Ⅰ), 6.5(Ⅱ), 15(Ⅲ), 30(Ⅳ), 60(Ⅴ)or 125(Ⅵ) mg/kg of supplemental Cu from Cu - amino acid. Beginning at week 10, blood and tissue samples were collected on week 12, 14 and 16. Result: As comparing with the control group (6.5 rng/kg), 30 mg/kg Cu - amino acid increases the index of thymus. 15 and 30 mg/kg Cu - amino acid increase the immune index of spleen and bursa of Fabri-cins, growth of lymphocyte, and the serum antibody value to New Castle disease. It is presumed that 15 ～ 30 mg/kg copper from Cu- amino acid is the optimal supplement dose for growing layers.