Application of supercritical fluid chromatography in the quantitative analysis of minor components (carotenes,vitamin E,sterols, and squalene) from palm oil |
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Authors: | Email author" target="_blank">Yuen?May?ChooEmail author Mei?Han?Ng Ah?Ngan?Ma Cheng?Hock?Chuah Mohd?Ali?Hashim |
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Affiliation: | (1) Persiaran Institusi, Malaysian Palm Oil Board (MPOB), No. 6, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia;(2) Department of Chemistry, University of Malaya, Kuala Lumpur, Malaysia;(3) Department of Chemical Engineering, University of Malaya, Kuala Lumpur, Malaysia |
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Abstract: | The application of supercritical fluid chromatography (SFC) coupled with a UV variable-wavelength detector to isolate the
minor components (carotenes, vitamin E, sterols, and squalene) in crude palm oil (CPO) and the residual oil from palm-pressed
fiber is reported. SFC is a good technique for the isolation and analysis of these compounds from the sources mentioned. The
carotenes, vitamin E, sterols, and squalene were isolated in less than 20 min. The individual vitamin E isomers present in
palm oil were also isolated into their respective components, α-tocopherol, α-tocotrienol, γ-tocopherol, γ-tocotrienol, and
δ-tocotrienol. Calibration of all the minor components of palm as well as the individual components of palm vitamin E was
carried out and was found to be comparable to those analyzed by other established analytical methods. |
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