丙型肝炎病毒3种标志物的稳定性

目的观察丙型肝炎病毒3种标志物的稳定性。方法用HCV-Ab、RT-PCR及HCV-Ag检测试剂分别检测血清样品中的HCV-Ab、HCV-RNA和HCV-Ag,比较3种标志物在不同温度、不同时间下保存及冻融后的稳定性。结果在2036份血清样品中,共检出HCV-Ab阳性样品85份、HCV-Ag阳性样品51份,HCV-RNA阳性样品12份;HCV-Ab4℃保存14d、-20℃保存3年及冻融5次稳定性良好,阳性率均为100%;HCV-RNA随保存时间的延长,阳性检出率逐步下降,-20℃保存3年后下降至33.33%,冻融后下降至83.33%;HCV-Ag-20℃保存3年后,阳性率下降至68.63%,4℃保存14d和冻融对其无影响。结论3种标志物中,HCV-Ab的稳定性最好,其次是HCV-Ag,HCV-RNA最不稳定。

Stability of Three Hepatitis C Virus Markers

Objective To observe the stability of three kinds of hepatitis C virus(HCV)markers. Methods The HCV-Ab, HCV-RNA and HCV-Ag in serum samples were determined by using HCV-Ab, RT-PCR and HCV-Ag kits respectively, based on which the stabilities of the 3 markers after storage at various temperatures for various times and after freeze-thawing were compared. Results Of the 2 036 samples, 85 were positive for HCV-Ab, 51 for HCV-Ag, and 12 for HCV-RNA. All the 85 HCV-Ab positive samples were still positive for HCV-Ab after storage at 4℃ for 14 d, at -20℃ for 3 years and after 5 cycles of freeze-thawing. However, only 33. 33% of 12 HCV-RNA positive samples were positive for HCV-RNA after storage at -20℃ for 3 years, and 83.33% after freeze-thawing. The HCV-Ag positive rate of 51 HCV-Ag positive samples decreased to 68. 63% after storage at -20℃ for 3 years, while showed no significant change after storage at 4℃ for 14 d or after freeze-thawing. Conclusion In the turn of stability, the three HCV markers were HCV-Ab, HCV-Ag and HCV-RNA.