抑制型离子色谱同时测定分离检测鱼酱油中的九种有机酸

建立了用亲水性阴离子交换分离柱,KOH为淋洗液作梯度淋洗,电导检测,同时分离和检测鱼酱油中九种有机酸的离子色谱法.方法对所测有机酸的线性范围均在2个数量级以上,回收率在93.65%～104.32%之间,该方法用于鱼酱油样品的分析结果显示具有准确、高效、回收率高和重现性好的特点.

Simultaneous Determination of Nine Kinds of Fish Sauce Organic Acids by Ion Chromatography with Suppressed Conductivity Detection

A method for the determination of nine kinds of fish sauce organic acids was developed by ion chromatography with suppressed conductivity detection.The separation of the organic acids Was achieved on hydrophilic anion exchange column with potassium hydroxide as gradient eluent with gradient pump.The detection Was performed by a conductivity detection mode. The calibrationgraphs 0f peal area for all analvtes were linear in the ranges about two orders of magnitude.The recoveries of addition standard ar ewithin93.65%～104.32%.The results in dicated that this method is highlv accurate and efficient with good reproducibility and high recovery.