| 聚阳离子海镁溴铵与DNA之间相互作用的光谱分析 |
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| 引用本文： | 廖杰,于冰,丛海林,刘虎威,曹维孝.聚阳离子海镁溴铵与DNA之间相互作用的光谱分析[J].现代仪器,2007,13(3):32-34. |
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| 作者姓名： | 廖杰 于冰 丛海林 刘虎威 曹维孝 |
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| 作者单位： | 1. 解放军总医院医学实验测试中心,北京,100853
2. 北京大学化学与分子工程学院,北京,100871 |
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| 摘 要： | 本文用紫外光谱、显微傅立叶变换红外光谱和圆二色光谱对聚阳离子海美溴铵(PB)和DNA之间的相互作用进行研究。以溴化乙锭作为与DNA结合的荧光探针,考察PB对其结合体系的影响。荧光分析结果显示,PB与DNA的相互作用可能对DNA二级结构的构型产生影响。IR光谱表明,PB通过磷酸基团和DNA碱基与DNA发生相互作用,DNA/PB复合物的形成引起DNA二级结构的构型变化,这一点也在CD光谱分析中得到验证。
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| 关 键 词： | 海美溴铵 DNA 相互作用 构型 光谱分析 |
Studies on the interaction between polybrene and DNA by spectral methods |
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| Liao Jie,Yu Bing,Cong Hailin,Liu Huwei,Cao Weixiao.Studies on the interaction between polybrene and DNA by spectral methods[J].Modern Instruments,2007,13(3):32-34. |
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| Authors: | Liao Jie Yu Bing Cong Hailin Liu Huwei Cao Weixiao |
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| Affiliation: | 1. Medical Experiment Analysis Center, PLA General Hospital, Bejing 100853;2. Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, 100871 |
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| Abstract: | The interaction of polybrene(PB) with hsDNA has been studied by spectroscopic analysis,microscopic IR-and CD-spectroscopy.Using ethidium bromide(EB) as a fluorescent probe,the binding of PB with DNA was studied.The fluorescence analysis shows that the interaction of DNA with PB may have effect on the conformation of secondary structure of DNA.IR-spectra show that PB interacts with DNA through both the phosphate groups and the bases of DNA and the formation of DNA/PB complex causes the change of the conformation of the DNA secondary structure,which is further proved by CD-spectra. |
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| Keywords: | DNA |
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