陕西平利绞股蓝氨基酸类成分指纹图谱研究

采用柱前衍生高效液相色谱（HPLC）技术对陕西平利绞股蓝中氨基酸类成分的指纹图谱进行研究。结果表明,以邻苯二甲醛为柱前衍生试剂,Kromasil C18分离,流动相为0.05mol/L醋酸钠+0.5%四氢呋喃-甲醇,采用梯度洗脱,绞股蓝中氨基酸类成分可得到良好的分离,同时对样品中16种氨基酸的含量进行定量;通过对13批样品HPLC图谱的分析,共确定22个色谱峰作为平利绞股蓝的特征指纹峰,利用夹角余弦法、相关系数法、中药色谱指纹图谱相似度评价系统软件进行相似度计算,13批陕西平利绞股蓝指纹图谱相似度0.989¹.00之间,该图谱能综合、准确地反映陕西平利绞股蓝氨基酸类成分的特征,为保护平利绞股蓝原产地域产品提供了新的途径。 

Study on HPLC fingerprints of amino acids constituents of Gynostemma pentaphylla cultivated pingli Shaanxi

Using high performance liquid chromatography (HPLC) with Pre-column Derivation technology to study the fingerpingt chromatogram of amino acids compounds of Gynostemma pentaphylla cultivated pingli Shaanxi. Results showed that the main amino acids compounds of Gynostemma could be separated well and determined with O-phthalaldehyde as the derivatization reagent, Kromasil C18column as the chromatographic column, 0.05mol/L sodium acetate with 0.5% furanidineand and methanol as the mobile phase, by the gradient elution method. 13 samples of HPLC fingerprints showed that this method could identify a total of 22chromatographic peaks as Gynostemma pentaphylla cultivated pingli Shaanxi fingerprint peaks. The similarities were determined by the coefficients of cosine, correlation and Similarity evaluation system for chromatographic fingerprint (Version 2004 A) . Results of similarity analysis were 0.989¹.00. It could generally and exactly reflect the characteristics amino acids compounds from pingli Shaanxi. The developed method provided a new method for protecting Gynostemma pentaphylla cultivated pingli source territory.