人参籽粕中水溶性多糖提取工艺优化

以脱脂人参籽粕为原料水提人参籽多糖。在乙醇浓度被确定的条件下,各个单因素实验的基础上,选取提取温度、提取时间以及料液比为自变量,多糖得率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖得率的影响。利用响应面分析方法,模拟得到二次多项式回归方程的预测模型,并确定水溶性人参籽多糖提取工艺的最佳条件为:提取温度93℃,提取时间为6.9h,料液比1∶25g/mL。在此条件下,人参籽多糖得率为7.59%。 

Optimization of extraction process of water-soluble polysaccharides from seeds meal of ginseng

Skim ginseng seed meal as raw, the water-soluble polysaccharide was extracted from ginseng seed.With the conditions determined in ethanol concentration, on the basis of single- factor experiment, selecting extraction temperature, extraction time and ratio of water to material as a variable, polysaccharide-yield as response values, central composite design methods were used to research the respective effects of polysaccharides of variables and their interaction on yield.The predictive polynomial quadratic equations model was developed by response surface analysis theory, and the optimal extraction conditions of the polysaccharides from seeds of ginseng were concluded as follows: extraction temperature 93℃, extraction time 6.9h and ratio of water to material 1 ∶ 25g /mL. Under these conditions, the yield of the polysaccharides was up to 7.59%.