高效液相色谱法测定芒果皮中脱落酸的含量

建立了高效液相色谱（HPLC）测定芒果果皮中脱落酸（ABA）含量的方法。确定了以无水乙醚为提取试剂,0.5%碳酸氢钠、乙酸乙酯和氯仿为萃取试剂的前处理过程,得到色谱条件为ODS-C18反相柱（4.6mm×250mm,5μm）,以甲醇和pH3.0乙酸溶液（60∶40,v/v）作为流动相,流速为0.8mL/min,检测器波长为252nm进行检测。结果表明,该方法标准曲线在0.50¹0.0mg/L范围内线性关系良好,R2=0.9996,平均回收率95.90%。该方法简单快速,为芒果采后ABA的生理作用研究提供了重要的技术支持。 

Analysis of abscisic acid content in peel of mango by HPLC

A HPLC method for determining abscisic acid (ABA) in peel of mango was established. The samples were homogenized in diethyl ether, partitioned by sodium carbonate solution, ethyl acetate and chloroform, separated on ODS-C18 column (4.6mm×250mm, 5μm) , eluted with the mixture of methanol and acetic acid solution at pH=3.0 (60∶40, v/v) at 0.8mL/min, and detected at 252nm. Results showed that the calibration curves of ABA had good linearity over the range from 0.50 to 10.0mg/L with a correlation coefficient of 0.9996. The average recovery rate for ABA was 95.90%. This method was simple and fast, which will provide technical support for the research of physiology function of ABA.