蘘荷红色素分离纯化及喷雾干燥工艺优化

研究分离纯化及喷雾干燥化蘘荷红色素的工艺条件。比较10 种大孔吸附树脂对红色素的静态、动态吸附与解吸效果，红色素纯化液经真空浓缩后用喷雾干燥法制备红色素的粉末。结果表明：LSA-21大孔吸附树脂分离纯化蘘荷红色素效果好，其工艺条件为上柱液pH 5.0、上样液质量浓度3.85 mg/mL、吸附流速2 BV/h，以酸性蒸馏水为解吸剂，解吸流速3 BV/h；喷雾干燥工艺条件为加入待喷液质量分数2%的可溶性淀粉，调整可溶性固形物质量分数达到 12%～14%、进料速率3.0～4.0 kg/h、进口风温度 174～177 ℃、出口风温度 65～71 ℃、排风速率 0.34～0.38 m3/min、雾化压强100 kPa。红色素粉末经薄层层析色谱与高效液相色谱检测均由3 种单体构成。

Separation and Purification by Macroporous Resin,and Spray Drying of Red Pigment from Zingiber mioga Rosc.

Objective: To investigate the separation and purification of red pigment from the bracts of Zingiber mioga Rosc.
and the preparation of red pigment powder by spray drying. Methods: Comparison of ten kinds of macroporous resin was
carried out for the static and dynamic adsorption and desorption of red pigment. The purified red pigment was vacuumconcentrated
before spray drying. Results: LSA-21 macroporous resin was found to be the best sorbent for the purification
of red pigment. The best results were obtained when the sample (3.85 mg/mL, pH 5.0) was loaded at a flow rate of 2 BV/h
and the adsorbed red pigment was eluted with acidified distilled water at a flow rate of 3 BV/h. The purified pigment solution
was added with 2% soluble starch and adjusted to a soluble solid content of 12%-14% prior to spray drying at a feeding
rate of 3.0-4.0 kg/h, an air inlet temperature of 174-177 ℃, an air outlet temperature of 65-71 ℃, an exhaust velocity of
0.34–0.38 m3/min, and an atomization pressure of 100 kPa. The resulting powder consisted of three monomers as detected by
high performance liquid chromatography (HPLC) and thin layer chromatograph (TLC). Conclusion: LSA-21 macroporous
adsorption resin is applicable for separation and purification of red pigment from Zingiber mioga Rosc. and the optimized
spray drying conditions are suitable for industrial production of red pigment powder from Zingiber mioga Rosc.