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| 响应面法优化红蚂蚁抗菌物质提取工艺 | |
| 引用本文： | 陈海燕,冯印,杨柳,尤丽新,陈晓平. 响应面法优化红蚂蚁抗菌物质提取工艺[J]. 食品科学, 2014, 35(22): 73-77. DOI: 10.7506/spkx1002-6630-201422014 |
| 作者姓名： | 陈海燕 冯印 杨柳 尤丽新 陈晓平 |
| 作者单位： | 1.长春科技学院生物食品学院，吉林 长春 130600；2.吉林农业大学食品科学与工程学院，吉林 长春 130118 |
| 基金项目： | 吉林省重大科技资助项目 |
| 摘 要： | 以长白山野生红蚂蚁为原料，提取红蚂蚁体内有效抗菌活性物质并进行抑菌效果评价。通过单因素试验和响应面分析法研究乙醇体积分数、料液比、离心转速对红蚂蚁提取物抑制大肠杆菌的影响，结果表明：最佳浸提条件为乙醇体积分数63%、料液比1∶26（g/mL）、离心转速7 230 r/min，对大肠杆菌抑菌圈直径为2.59 cm。 |
| 关 键 词： | 响应面法 红蚂蚁 抗菌物质 |
Optimization of Extraction of Antibacterial Compounds from Red Ants with Response Surface Methodology |
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| CHEN Hai-yan,FENG Yin,YANG Liu,YOU Li-xin,CHEN Xiao-ping. Optimization of Extraction of Antibacterial Compounds from Red Ants with Response Surface Methodology[J]. Food Science, 2014, 35(22): 73-77. DOI: 10.7506/spkx1002-6630-201422014 | |
| Authors: | CHEN Hai-yan FENG Yin YANG Liu YOU Li-xin CHEN Xiao-ping |
| Affiliation: | 1. College of Biotechnology and Food Science, Changchun University of Science and Technology, Changchun 130600, China;2. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China |
| Abstract: | Antibacterial compounds were extracted from wild red ants from the Changbai Mountains and their antibacterial activity was evaluated. The anti-E. coli activity as a function of ethanol concentration, solid-to-solvent ratio and centrifugal speed was investigated using response surface methodology. The diameter of the inhibition zone against E. coli was 2.59 cm under the optimal extraction conditions: 63% aqueous ethanol, a solid-to-solvent ratio of 1:26 (g/mL) and centrifugation at a speed of 7 230 r/min. |
| Keywords: | response surface methodology red ant antimicrobial compounds |
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