乙醇-硫酸铵双水相体系萃取坛紫菜多糖

探究不同系线长度的乙醇-硫酸铵双水相体系对坛紫菜多糖萃取的影响。采用浊点滴定法绘制乙醇-硫酸铵双水相相图，选取不同系线长度体系，测定其对紫菜多糖分配情况。结果表明：在乙醇质量分数27%、硫酸铵质量分数18%（即系线长度为40）、体系相比R为1.1时，紫菜多糖的回收率可达79.8%，分配系数K为0.23；其蛋白去除率明显高于传统醇沉法（双水相萃取多糖蛋白含量为1.20%，醇沉多糖蛋白含量为6.17%），且具有较好的脱色效果，可用于紫菜多糖的分离纯化。

Extraction of Polysaccharides from Porphyra haitanensis Using Ethanol-Ammonium Sulfate Aqueous Two-Phase System

The effect of tie line length (TLL) of ethanol-(NH4)2SO4 aqueous two phase system (ATPS) on the extraction
of polysaccharides from Porphyra haitanensis was investigated. The phase diagram was obtained by cloud point titration,
and then different TLLs of ATPS were selected and partition coefficients of polysaccharides were detected. The yield
and partition coefficient of polysaccharides were 79.8% and 0.23, respectively, using a system consisting of 27% ethanol
and 18% (NH4)2SO4 (forming a TLL of 40) with a top/bottom phase volume ratio of 1.1. The removal rate of protein was
significantly higher than that reported with traditional ethanol precipitation method (1.20% vs 6.17%). Meanwhile, the
proposed method had good decolorization effect. Thus, it can be used for the separation and purification of polysaccharides
from Porphyra haitanensis.