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Green recovery of phenolic compounds from rice byproduct (rice bran) using glycerol based on viscosity,conductivity and density
Authors:Hao Huang  Zhenni Wang  Halah Aalim  Jarukitt Limwachiranon  Li Li  Zhenhua Duan  Guoping Ren  Zisheng Luo
Affiliation:1. College of Biosystems Engineering and Food Science, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agri-Food Processing, Zhejiang University, Hangzhou, 310058 China;2. Institute of Food Science and Engineering, Hezhou University, Hezhou, China;3. Hangzhou Wanxiang Polytechnic, Huawu Road 3, Hangzhou, 310023 China
Abstract:Rice bran, the industry byproduct, contains high levels of phenolic compounds. Based on glycerol, a green recovery of phenolics from rice bran was proposed. Extraction temperature (X1), glycerol concentration (X2) and liquid-to-solid ratio (X3) were optimised by response surface methodology. Basic physical properties, that is, viscosity, conductivity, density were analysed and UPLC-Triple-TOF/MS method was applied for phenolic identification. Results showed the optimum conditions were X1 = 66.76 °C, X2 = 19.47% and X3 = 32.92 mL g−1. Under these conditions, the recovery yield in total polyphenols was 708.58 ± 12.36 mg GAE/100 g DW. Moreover, the recovery capacity of phenolic compounds was significantly correlated with viscosity, conductivity and density of glycerol solution. Under the optimal conditions, totals of thirteen phenolic compounds were identified and phenolic acids (4-hydroxybenzoic acid, vanillic acid, syringic acid, 4-coumaric acid, ferulic acid and salicylic acid) were quantified. These results demonstrate glycerol can serve as an ideal green solvent to recover phenolic compounds from rice bran.
Keywords:Glycerol  green extraction  optimisation  phenolics  physical properties  rice bran
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