坛紫菜单性组织培养的性逆转研究

为了进一步研究坛紫菜的单性组织发育特征并验证其可能存在的单性生殖途径，本文对坛紫菜雌、雄营养细胞、组织发育过程中两性生殖组织的形成和繁殖特征以及两种单性遗传来源的子代叶状体的性别发育特征进行观察研究．结果表明，单性营养细胞和组织能够通过性逆转产生两性生殖组织并形成丝状体，该繁殖方式不属于单性生殖．单性遗传来源的子代叶状体群体由于性逆转产生了雌性、雄性以及雌雄同体三种性别表现．这种性逆转现象在紫菜属植物中尚未见报道．坛紫菜性逆转现象的发现为育种过程中丝状体纯系的分离以及性别发生机制的研究提供了新的理论借鉴．

Sex Reversal of Porphyra haitanensis Starting from the Unisexual Thalloid Tissue Culture

In order to ascertain more about development ofPorphyra haitanensis and investigate whether P. haitanensis can reproduce by parthenogenesis under the unisexual culture condition. Some developmental features were observed during the development of the male and female vegetative cells and tissues, including the formation of reproductive tissues and their reproductive patterns. The results show that the unisexual cells and tissues can generate reproductive cells of both sex by sex reversal and form conchocelis. That is different from parthenogenesis in which the ovum develops into a new individual without fertilization. Two F1 young blade populations derived from the two conchocelis were investigated for their sexual expression, the results show that both F1 blade populations have male, female and hermaphrodite individuals because of sex reversal in some individuals. It is not found to be reported on the existing of sex reversal in life history ofporphyra. The find- ings have important significance to isolation of genetic pure strains in breeding and study on sexual development mechanism.