| 高效液相色谱法快速测定海产品中牛磺酸 |
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| 引用本文： | 张晾,耿越,张静静,涂文利. 高效液相色谱法快速测定海产品中牛磺酸[J]. 食品与药品, 2006, 8(4): 56-58 |
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| 作者姓名： | 张晾 耿越 张静静 涂文利 |
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| 作者单位： | 山东师范大学生命科学学院动物抗性重点实验室,山东,济南,250014 |
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| 基金项目： | 山东省青年科学家科研奖励基金 |
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| 摘 要： | 目的建立用高效液相色谱快速测定海产品中牛磺酸的方法。方法将长竹蛏去壳,经匀浆、水煮、离心后用磺基水杨酸沉淀蛋白,采用邻苯二甲醛柱前衍生,通过EclipseZorbaxC18柱进行分离、330nm波长测定牛磺酸含量。结果此法平均回收率为101.3%,精密度、灵敏度较高,其它成分不干扰本测定。结论此法可以快速测定海产品中的牛磺酸。
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| 关 键 词： | 高效液相色谱 柱前衍生 牛磺酸 长竹蛏 |
| 文章编号： | 1672-979X(2006)04-0056-03 |
| 修稿时间： | 2005-11-20 |
Fast determination of taurine in marine products by high performance liquid chromatography |
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| ZHANG Liang,GENG Yue,ZHANG Jing-jing,TU Wen-li. Fast determination of taurine in marine products by high performance liquid chromatography[J]. Food and Drug, 2006, 8(4): 56-58 |
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| Authors: | ZHANG Liang GENG Yue ZHANG Jing-jing TU Wen-li |
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| Abstract: | Objective To establish the method for fast determination of taurine in marine products by high performance liquid chromatography(HPLC).Methods Solens were shelled,then homogenized,boiled and centrifugated.Proteins in solution were deposited by 5-sulfosalicylic acid.The content of taurine in solen was determined by high performance liquid chromatography at the wavelength of 330 nm through Eclipse Zorbax C18 column with pre-column opthaldialdehyde derivatization.Results The proposed method had high precision and sensitivity and was not interfered by other complex components.The average recovery was 101.3%.Conclusion This method can be used for fast determination of taurine in marine products. |
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| Keywords: | High Performance liquid chromatography Pre-column derivatization Taurine Solen gouldii |
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