自上而下法评定固相酶联免疫吸附法测定食用油中黄曲霉毒素B1的不确定度

目的利用自上而下方法评定固相酶联免疫吸附法测定食用油中黄曲霉毒素B_1含量的不确定度。方法在控制不确定度来源或程序的前提下,得出试验数据、质控数据进行评定,通过计算偏移和实验室内复现性2个分量,最后合成得到该方法的测量不确定度。结果按照自上而下法的公式计算,本研究测得食物油中黄曲霉毒素B_1含量为(46.25±6.28)μg/m L,扩展不确定度U_(rel)为14%(k=2)。结论使用自上而下法对固相酶联免疫吸附法测定食用油中黄曲霉毒素B_1含量进评定过程是实用和可靠的,可以为其他同类型的检测方法进行测量不确定度评定时所借鉴,也为实验员在实验质量控制方面提供参考。

Uncertainty evaluation of determination of aflatoxin B1 in edible oil by ELISA with top-down approach

The aim of this paper was to evaluate the uncertainty of Aflatoxin B1 in edible oil by ELISA with Top-down approach. The method was based on the control of the source or procedure of uncertainty, the test data and QC data were evaluated , by calculating the two components that was offset and laboratory reproducibility of, The measurement uncertainty of the method is obtained by synthesis in the final. The result is that the relative uncertainty of the measurement is 0.26 according to the top-down method, regardless of the contribution of the various components to the measurement uncertainty before and after the measurement, the conclusion is that the top-down approach determination of the content of aflatoxin B1 in edible oil by ELISA method is practical and reliable. It can be used for the measurement of other similar types of detection methods, and also for the experimental quality control for reference.