应用间接ELISA技术快速检测花鲈病原菌-鳗弧菌

以花鲈（Lateolabrax japonicus)弧菌病的病原菌－鳗弧菌（Vibrio auguillarum)W-1为抗原，制备兔抗血清；利用辣根过氧化酶标记的羊抗兔血清（HRP－IgG)为酶标二抗，建立了检测鳗弧菌的间接ELISA快速检测法。结果表明，应用间接ELISA技术检测鳗弧菌有较高的灵敏度，最低的检测量为10^5个／ml，即10^4个／孔。同时交叉反应表明其具有较高的特异性。对46份人工感染后的花鲈组织样品，包括肌肉、鳃、肠、肝、肾等组织的检测表明，阳性检测率为76．1％。

An Indirect ELISA Technique for Rapid Detection of Vibrio anguillarum in Sea perch (Lateolabrax japonicus

An indirect enzyme linked immunosorbent assays (ELISA) was developed for the rapid detection of Vibrio anguillarum in sea perch ( Lateolabrax japonicus ) which infected artificially. The ELISA, which incorporated a polyclonal antiserum produced in a male New Zealand white rabbit, detected 10 5 cells of V. anguillarum /ml. The systems permitted the recognition of V. anguillarum isolates, but not those of other taxa. The specificity and sensitivity antiserum were test on tissues which including muscle, hinder gut, gill, liver and kidney from sea perch infected artificially with viable W 1 ( V. anguillarum ).The results showed 76.1% of 46 samples were positive by indirect ELISA detection.