Direct colorimetric monoclonal antibody enzyme immunoassay for estradiol-17 beta in saliva |
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Authors: | K Tamate M Charleton JP Gosling D Egan M Ishikawa PF Fottrell MM Kane |
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Affiliation: | National Diagnostics Centre-Bioresearch Ireland, University College, Galway, Ireland. |
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Abstract: | We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17 beta in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17 beta-6-carboxymethyloximebovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B0-3 SD) is 365 amol/well or 7.3 pmol/L when 50-microL samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is < 0.2%. Estradiol-17 beta was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in approximately 3 h and may be useful for fertility monitoring and management. |
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