| 荧光寿命成像技术及其研究进展 |
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| 引用本文： | 刘超,周燕,王新伟,刘育梁. 荧光寿命成像技术及其研究进展[J]. 激光与光电子学进展, 2011, 0(11) |
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| 作者姓名： | 刘超 周燕 王新伟 刘育梁 |
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| 作者单位： | 中国科学院半导体研究所光电系统实验室; |
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| 摘 要： | 荧光寿命显微成像(FLIM)技术是一种新颖的荧光成像技术,具有其他荧光成像方法无法替代的优异性能,是生物医学工程领域的研究热点。频域调制、门控探测和时间相关单光子计数(TCSPC)是FLIM的几种主要实现方法。综述了这些技术的原理、研究现状和已取得的部分成果,比较了这三种方法的时间分辨率和成像速度等参数的优劣。宽场FLIM更适用于延时成像和实时成像。荧光偏振各相异性成像和内窥镜FLIM技术都是FLIM技术很有前景的应用方向。
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| 关 键 词： | 测量 荧光寿命成像 单光子计数 门控探测 频域调制 |
Fluorescence Lifetime Imaging Microscopy and Its Research Progress |
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| Liu Chao Zhou Yan Wang Xinwei Liu Yuliang. Fluorescence Lifetime Imaging Microscopy and Its Research Progress[J]. Laser & Optoelectronics Progress, 2011, 0(11) |
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| Authors: | Liu Chao Zhou Yan Wang Xinwei Liu Yuliang |
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| Affiliation: | Liu Chao Zhou Yan Wang Xinwei Liu Yuliang(Optoelectronic System Laboratory,Institute of Semiconductors,Chinese Academy of Sciences,Beijing 100083,China) |
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| Abstract: | Fluorescence lifetime imaging microscopy(FLIM) technology has so many outstanding and unique advantages compared with other fluorescence imaging methods that it attracts more and more researchers in biomedicine engineering field.Frequency domain modulation method and time domain methods including time-gated detection and time correlated single photon counting(TCSPC),are the main ways to realize FLIM.Principles,current reaseach situation and achievements of these methods are presented as well as the comparis... |
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| Keywords: | measurement fluorescence lifetime imaging microscopy time-correlated single photon counting time-gated detection frequency modulation |
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