一种应用比率荧光法定量分析水体中亚甲基蓝含量的方法

亚甲基蓝作为一种染料，被广泛用于工业、医药、水产养殖和皮肤护理等领域.但是过量使用亚甲基蓝，不仅污染环境，还会危害人类和动物的健康.通过将石墨烯量子点与CdTe量子点混合得到比率荧光检测体系，依据荧光强度变化和亚甲基蓝浓度之间的线性关系，建立了一种定量分析亚甲基蓝含量的方法，线性范围为3.13～9.38 nmol·L^-1,最低检出限为0.558 9 nmol·L^-1.该方法具有较高的灵敏性、选择性与稳定性，可用于检测水溶液中亚甲基蓝含量.

A Ratio-Fluorescence Method for Quantitative Analysis of Methylene Blue

Methylene blue, as a dye, has been widely used in industry, medicine, aquaculture, skin care, and et al. But excessive use of methylene blue, not only pollutes the environment, but also endangers the health of human beings and animals. In this paper, ratio-fluorescence detection system has been founded through mixing of graphene and CdTe quantum dots, and a method for quantitative analysis of MB is founded based on linear relationship between variety of fluorescence intensity and concentrations of methylene blue, of which linear range is 3.13 - 9.38 nmol/L and the lowest detection limit is 0.5589 nmol/L. This method have good sensitivity, selectivity and stability, could be used for more accurate detection of methylene blue.