Inexpensive holder for hand-trimming resin-embedded tissues |
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Authors: | Wise Robert R |
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Affiliation: | Department of Biology, University of Wisconsin-Oshkosh, Oshkosh, Wisconsin 54901, USA. wise@uwosh.edu |
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Abstract: | A simple and inexpensive mount for securely holding resin-embedded tissues for trimming prior to sectioning for light or electron microscopy is described. The unit is composed of a base plate from which a 12.7-mm drill chuck protrudes. A tissue block is clamped into a microtome chuck and that is mounted in the drill chuck. A standard dissecting microscope is placed over the unit and used to magnify the block for trimming. Total cost of materials is estimated to be $20-40 USD. |
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Keywords: | TEM specimen preparation hand trimming |
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