离子液体[BMIM]Cl处理汽爆活化玉米秸秆及酶解

为了提高玉米秸秆酶解还原糖产率,利用蒸汽爆破法活化玉米秸秆原料,并利用离子液体[BMIM]Cl进行处理,考察了汽爆压力和维压时间对处理后物料酶解还原糖产率的影响。结果表明,汽爆压力2.6 MPa,维压时间90 s下汽爆活化秸秆原料,[BMIM]Cl处理后,酶解24 h后还原糖产率较汽爆活化后物料提高了84.03%,较原料提高了286.83%。秸秆化学组分分析表明,[BMIM]Cl处理后物料纤维素质量分数增加了64.86%,X射线衍射(XRD)与扫描电镜(SEM)分析表明,其晶形结构转变为无定形结构,更有利于纤维素酶与底物作用。说明汽爆活化[BMIM]Cl处理能显著提高玉米秸秆的酶解还原糖产率。

Enzymatic hydrolysis of corn stalk treated by ionic liquid [BMIM]Cl after steam explosion activation

For improving the yield of reduced sugars from corn stalk enzymatic hydrolysis,the steam explosion was selected as an activation method,and ionic liquid [BMIM]Cl was used as a further treatment step.The influence of steam explosion pressure and time on the yield of reduced sugars was evaluated.The results show that,after being activated with a steam pressure of 2.6 MPa for 90 seconds′ treatment,the yield of reduced sugars from [BMIM]Cl treated corn stalk can increase by 84.03% compared to that from activated corn stalk and by 286.83% compared to that from untreated corn stalk.The relative content of cellulose in [BMIM]Cl treated corn stalk increased by 64.86%.X-ray diffraction analysis(XRD) and Scanning electronic microscopy(SEM) indicate that the structure of [BMIM]Cl treated corn stalk became amorphous.The changes of structure led to easier attachment of the cellulose.Thus the conclusion is drawn that the treatment by [BMIM]Cl and activation by steam explosion can significantly increase the yield of reduced sugars from corn stalk.