响应曲面法优化超声辅助提取油菜籽皮中的原花青素

以乙醇为提取剂,用超声辅助的方法从油菜籽皮中提取了原花青素。在讨论了提取剂体积分数、提取时间、液料比(每克原料加入提取剂的毫升数,简称液料比,以下同)和超声波功率等单因素实验的基础上,运用Box-Behnken中心组合实验和响应曲面法分析了提取剂体积分数、提取时间和液料比3个因素对原花青素提取率的影响,并优化了提取工艺。结果表明,最佳的工艺条件为:乙醇体积分数62.9%,提取时间20.8 min,液料比25 mL/g。在该条件下,原花青素提取率的预测值为5.055 mg/g,验证实验值为5.02 mg/g,说明响应曲面法优化油菜籽皮中原花青素提取工艺是可行的。

Optimization of ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using response surface methodology

Ultrasound assisted extraction of proanthocyanidin from rapeseed hull using ethanol as the extraction solvent was studied. Based on the single factor tests of ethanol volume fraction, extraction time, liquid-solid ratio and ultrasound power, the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. The results indicated that ethanol volume fraction 62.9%, extraction time of 20.8min and liquid-solid ratio of 25mL/g were found optimum. Under these optimized conditions, the experimental value of proanthocyanidin extraction yield 5.05mg/g, in good agreement with the predictive value 5.02mg/g. Therefore, the ultrasound-assisted extraction of proanthocyanidin from rapeseed hull using response surface methodology is very reliable.