| 红曲霉M9x的原生质体形成与再生条件的研究 |
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| 引用本文： | 戴德慧,蒋家新,黄光荣.红曲霉M9x的原生质体形成与再生条件的研究[J].食品科学,2006,27(1):97-100. |
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| 作者姓名： | 戴德慧 蒋家新 黄光荣 |
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| 作者单位： | 浙江科技学院生化系 |
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| 摘 要： | 对红曲霉M9x用混合酶制取原生质体及原生质体再生条件进行了探索。其原生质形成和再生最佳条件为:以豆芽汁培养液为菌丝培养基,采用60h菌龄的菌丝体,以0.6mol/LMgSO4作为渗稳剂,用1.5%混合酶(蜗牛酶:纤维素酶=7:3)在30℃处理2h,原生质体形成数可以达到1.61×107个/ml,再生率为8.49%。
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| 关 键 词： | 红曲霉 原生质体 形成 再生 |
| 文章编号： | 1002-6630（2006）01-0097-04 |
| 收稿时间： | 2004-11-23 |
| 修稿时间： | 2004-11-23 |
Study on Formation and Regeneration of the Protoplast from Monascus ruber M9x |
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| DAI De-hui,JIANG Jia-xin,HUANG Guang-rong.Study on Formation and Regeneration of the Protoplast from Monascus ruber M9x[J].Food Science,2006,27(1):97-100. |
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| Authors: | DAI De-hui JIANG Jia-xin HUANG Guang-rong |
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| Affiliation: | School of Biological and Chemical Engineering, Zhejiang University of Science and Technology |
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| Abstract: | The formation and regeneration of the protoplast from Monascus ruber M9x were studied. The results showed that the soybean sprout juice used to culture mycelium and the mycelium aged 60h used as operated material were easily processed to form protoplast. Under the action of 1.5% mixed enzyme (snail enzyme: cellulose enzyme =7:3) and with 0.6mol/L MgSO4 used as osmotic stabilizer, the protoplasts could reach 1.61×1 07/ml, and the regeneration rate was 8.49%. |
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| Keywords: | Monascus ruber protoplast formation regeneration |
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