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植物超弱发光的探测
引用本文:王维江 邢达. 植物超弱发光的探测[J]. 光电子.激光, 2000, 11(4): 427-430
作者姓名:王维江 邢达
作者单位:[1]广东工业大学应用物理系 [2]华南师范大学激光生命科学研究所
摘    要:采用超弱发光图像探测系统对蟛蜞菊、千日红花蕾、翠菊等植物样品进行了自发和光诱导的超弱发光图像探测,对大花紫薇叶片的延迟发光进行了初步的光谱探测。结果表明:植物自发发光与延迟发光的强度及其分布是有区别的,自发光光强度远小于延迟发光强度;与有丝分裂和生长代谢有关的植物超弱发光有光诱导下得以加强;千日红花蕾的延迟发光遵循双曲线衰减规律,说明生物光子具有一定的相干性;叶片的延迟发光光谱比离体叶绿素荧光光谱

关 键 词:超弱发光 图像探测 植物样品
修稿时间:2000-05-11

Detection of Plant Ultraweak Bioluminescence
WANG Wei-jiang,XING D,TAN Shi-ci,HAN Jun- ying. Detection of Plant Ultraweak Bioluminescence[J]. Journal of Optoelectronics·laser, 2000, 11(4): 427-430
Authors:WANG Wei-jiang  XING D  TAN Shi-ci  HAN Jun- ying
Affiliation:WANG Wei-jiang 1,XING Da 2,TAN Shi-ci 2,HAN Jun- ying 3
Abstract:Using an ultraweak luminescent image d etecting system,the spontaneous and delayed bioluminescent images of Wedelia,Gom phrena globosa bud and callistephus Chinensis are detected.Furthermore,a prelimi nary spectrum detection of Lagerstroemia speciosa lamina is done.Following are t he main results.The intensity and its distribution of plant spontaneous biolumin escence (SL) are different from that of delayed bioluminescence (DL).Generally,t he intensity of SL is much lower than that of DL.The ultraweak bioluminescence r elated to the mitosis and metabolism of a plant sample is strengthened after the sample is illuminated by light.The measured photon count rate of a Gomphrena gl obosa bud after light illumination follows a hyperbolic decay law,indicating the coherence of biophoton emission.The DL spectrum of Lagerstroemia speciosa lamin a is more complex than the fluorescence spectrum of exsomatize chlorophyll and t he former contains more physiological message. -
Keywords:ultraweak bioluminescence  wedelia  g omphrena globosa L  callistephus chinensis nees  lagerstroemia speciosa (L) pers  spectrum  image intensifier
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