| 反相高效液相色谱法测定肾康口服液中黄柏的小檗碱含量 |
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| 引用本文： | 刘腊娥,陈立新,梁爱君,王绪明.反相高效液相色谱法测定肾康口服液中黄柏的小檗碱含量[J].福建分析测试,2005,14(1):2123-2125. |
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| 作者姓名： | 刘腊娥 陈立新 梁爱君 王绪明 |
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| 作者单位： | 1. 中国人民解放军第163医院药剂科,湖南,长沙,4100032
2. 中国人民解放军总后勤部药品仪2S检验所,北京,100071 |
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| 摘 要： | 建立反相高效液相色谱法测定肾康口服液中黄柏的小檗碱含量。采用反相高效液相色谱法,Novapak C18柱(3.9×150mm,5μm)流动相为甲醇一乙腈(0.05mol·L-1)-磷酸二氢钠溶液(20∶3S∶45),流速为1.0mL·min-1,检测波长为346nm。小檗碱的回归方程为C=1.2616+1821X10-6A,r=0.9992,在4.00~20.00μg·mL-1范围内呈线性关系。平均回收率和RSD分别为101.32%和1.18%(n=5)。方法操作简便,测定结果准确可靠,可用于测定肾康口服液中黄柏的小檗碱含量。
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| 关 键 词： | 反相高效液相色谱法 肾康口服液 小檗碱 |
Determination on berberine of Huangbo in Shenkang oral liquid by RP-HPLC |
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| Liu Lae,Chen Lixin,Liang Aijun,WANG Xuming.Determination on berberine of Huangbo in Shenkang oral liquid by RP-HPLC[J].Fujian Anal Ysis & Testing,2005,14(1):2123-2125. |
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| Authors: | Liu Lae Chen Lixin Liang Aijun WANG Xuming |
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| Abstract: | A quantitative method was developed for the determination on berberine of Huangbo in Shengkang oral liquid by RP-HPLC.Thechromatographic conditions include column Novapak C_ 18(3.9×150mm,5μn),mobile phase:methanol-methylcyanide(0.05mol·L -1-sodium dihydrogen phosphate(20∶35∶45),flow rate1.omL/ min,detection waVelength 346nm.The regression equation of berberine hydrochloride was C=1.2616+8.1821×10 -6A r-0.9992. The standard curve was linear in the range of 4.00～20.00 μg·mL -1. The average added recovery and RSD were 101.32%and 1.18% (n=5),respeetively. The method was simple sensitive,rapid and accurate.It may be used for determination of berberine in Huanbo of shenkang oral liquia. |
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| Keywords: | RP-HPLC Shengkang oral liquid Berberine |
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