烟酰胺和3-氰基吡啶的HPLC检测

采用高效液相色谱法测定烟酰胺和3-氰基吡啶的含量.色谱条件为:STR-ODSⅡ柱(4.6 mm×150 mm),流动相为甲醇∶水=1 ∶ 3,流速1.000 mL·min-1,检测波长254 nm,柱温30℃.该方法的烟酰胺和3-氰基吡啶检测范围均为1.0～40.0 mg·L-1,烟酰胺的检测限为0.25 mg·L-1...

Determination of Nicotinamide and 3-Cyanopyridine by High Performance Liquid Chromatography

The simultaneous determination of nicotinamide and 3-cyanopyridine with the high performance liquid chromatographic method was described.The conditions of chromatography were as follows:Using column STR-ODSⅡ(4.6 mm×150 mm),mobile phase of methanol∶water=1∶3,flow rate of 1.000 mL·min-1,detection wavelength of 254 nm,column temperature of 30 ℃.The detection range of nicotinamide and 3-cyanopyridine were from 1.0 mg·L-1 to 40.0 mg·L-1.The detection limit of nicotinamide and 3-cyanopyridine was 0.25 mg·L-1,1.09 mg·L-1,respectively.The RSD(n=5) of nicotinamide and 3-cyanopyridine was 2.1%~3.8%,0.9%~3.1%,respectively.This method is rapid,accurate with high repreducibility.