| 反胶束体系中固定化胃蛋白酶的催化性质研究 |
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| 引用本文： | 伏振宇,李志光,何纯莲. 反胶束体系中固定化胃蛋白酶的催化性质研究[J]. 化学与生物工程, 2011, 28(10): 75-78. DOI: 10.3969/j.issn.1672-5425.2011.10.020 |
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| 作者姓名： | 伏振宇 李志光 何纯莲 |
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| 作者单位： | 1. 湖南农业大学理学院,湖南长沙,410128
2. 湖南师范大学医学院,湖南长沙,410006 |
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| 摘 要： | 将胃蛋白酶加至CTAB(十六烷基三甲基溴化铵)/环己烷/正辛醇反胶束体系中得到固定化胃蛋白酶,研究了反胶束含水率、乙醇体积分数对固定化胃蛋白酶活力的影响,并对固定化胃蛋白酶和游离胃蛋白酶的催化性质进行了比较研究.结果表明,反胶束含水率为12％、乙醇体积分数为30％时,固定化胃蛋白酶的活力达到最佳；固定化胃蛋白酶和游离胃...
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| 关 键 词： | 胃蛋白酶 CTAB(十六烷基三甲基溴化铵) 反胶束 酶活力 |
Research on Catalytic Property of Immobilized Pepsin in Reverse Micelles |
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| FU Zhen-yu,LI Zhi-guang,HE Chun-lian. Research on Catalytic Property of Immobilized Pepsin in Reverse Micelles[J]. Chemistry & Bioengineering, 2011, 28(10): 75-78. DOI: 10.3969/j.issn.1672-5425.2011.10.020 |
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| Authors: | FU Zhen-yu LI Zhi-guang HE Chun-lian |
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| Affiliation: | FU Zhen-yu1,LI Zhi-guang1,HE Chun-lian2(1.College of Science,Hunan Agricultural University,Changsha 410128,China,2.College of Medicine,Hunan Normal University,Changsha 410006,China) |
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| Abstract: | Pepsin was solubilized in reverse micelles of CTAB(cetyltrimethylammonium bromide) in cyclohexane with octanol as co-surfactant.The effects of water content and ethanol volume fraction of reverse micells on immobilized pepsin activity were studied.And the catalytic properties of free and immobilized pepsin were investigated.The results indicated that the enzymatic activity of immobilized pepsin was the best when water content was 12%,ethanol volume fraction was 30%;the optimum temperature of immobilized and... |
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| Keywords: | pepsin CTAB(cetyltrimethylammonium bromide) reverse micelles enzymatic activity |
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