| HPLC法同时测定发酵液中纽莫康定Ao与Bo的含量 |
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| 引用本文： | 张雪霞,李宁,李晓露,林旸,张金娟,林毅,蒋沁. HPLC法同时测定发酵液中纽莫康定Ao与Bo的含量[J]. 化学与生物工程, 2011, 28(8): 88-90. DOI: 10.3969/j.issn.1672-5425.2011.08.024 |
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| 作者姓名： | 张雪霞 李宁 李晓露 林旸 张金娟 林毅 蒋沁 |
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| 作者单位： | 微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,华北制药集团新药研究开发有限责任公司,河北石家庄050015 |
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| 基金项目： | 国家“重大新药创制”科技专项(2010ZX09401-403) |
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| 摘 要： | 采用HPLC方法同时测定发酵液中纽莫康定A0与B0的含量.色谱柱为SepaxSapphire C18 (4.6 mm×250mm,5 μm),流动相为甲醇-水(体积比45:55,内含0.1‰三氟乙酸),流速为1.0 mL·min-1,紫外检测波长为214nm.结果表明,纽莫康定A0与B0的浓度分别在47.5～191.4...
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| 关 键 词： | 高效液相色谱法 纽莫康定A0 纽莫康定BD |
Simultaneous Determination of Content of Pneumocandin A_0 and B_0 in Fermentation Broth by HPLC |
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| ZHANG Xne-xia,LI Ning,LI Xiao-lu,LIN Yang,ZHANG Jin-iuan,LIN Yi,JIANG Qin. Simultaneous Determination of Content of Pneumocandin A_0 and B_0 in Fermentation Broth by HPLC[J]. Chemistry & Bioengineering, 2011, 28(8): 88-90. DOI: 10.3969/j.issn.1672-5425.2011.08.024 |
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| Authors: | ZHANG Xne-xia LI Ning LI Xiao-lu LIN Yang ZHANG Jin-iuan LIN Yi JIANG Qin |
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| Affiliation: | ZHANG Xue-xia,LI Ning,LI Xiao-lu,LIN Yang,ZHANG Jin-juan,LIN Yi,JIANG Qin(National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Metabolic Engineering & Technology Research Center,New Drug Research & Development Company of NCPC,Shijiazhuang 050015,China) |
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| Abstract: | Simultaneous determination of content of pneumocandin A0 and B0 in fermentation broth by HPLC was studied.The HPLC conditions consisted of a SepaxSapphire C18 column(4.6 mm×250 mm,5 μm),mobile phase of methanol-H2O(45∶55) containing 0.1‰ trifluoracetic acid with a flow rate of 1.0 mL·min-1 and detection wavelength at 214 nm.The linearity of pneumocandin A0 and B0 concentration was shown in the range of 47.5～191.4 μg·mL-1(R=0.9997) and 38.4～153.6 μg·mL-1(R=0.9998);the sensitivity of pneumocandin A0 and B0 was 6.86 ng,5.95 ng;the average recovery of pneumocandin A0 and B0 was 99.15% with RSD=0.96%(n=9) and 99.31% with RSD=0.98%(n=9),respectively.The HPLC method,which was simple and accurate with good repeatability,could be applied to simultaneous determination of content of pneumocandin A0 and B0 in fermentation broth. |
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| Keywords: | high performance liquid chromatography(HPLC) pneumocandin A0 pneumocandin B0 |
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