| 微生物细胞脂肪酸的组成分析 |
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| 引用本文： | 柳志杰,章辉,周利,花强.微生物细胞脂肪酸的组成分析[J].化学与生物工程,2011,28(10):85-87. |
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| 作者姓名： | 柳志杰 章辉 周利 花强 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237 |
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| 基金项目： | 上海市浦江人才计划项目(08PJ14038); 国家重点实验室专项经费资助项目(2060204); 教育部留学回国人员科研启动基金资助项目; 上海市重点学科建设项目(B505) |
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| 摘 要： | 应用气相色谱—质谱联用技术(GC-MS)对微生物细胞脂肪酸的组成进行了分析.结果表明,菌体的代谢状态分为两个时期:菌体生长期和油脂积累期；菌体细胞主要积累油酸(C18:1)、软脂酸(C16:0)和棕桐油酸(C16:1);通过发酵,最终得到的微生物油脂脂肪酸组成为:油酸(C18:1)64.80％、软脂酸(C16:0)19...
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| 关 键 词： | 微生物细胞 油脂含量 脂肪酸组成 气相色谱—质谱联用技术 |
Analysis of Composition of Microorganism Cellular Fatty Acid |
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| LIU Zhi-jie,ZHANG Hui,ZHOU Li,HUA Qiang.Analysis of Composition of Microorganism Cellular Fatty Acid[J].Chemistry & Bioengineering,2011,28(10):85-87. |
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| Authors: | LIU Zhi-jie ZHANG Hui ZHOU Li HUA Qiang |
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| Affiliation: | LIU Zhi-jie,ZHANG Hui,ZHOU Li,HUA Qiang(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China) |
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| Abstract: | The composition of microorganism cellular fatty acid was analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that the metabolism of the strain could be divided into cell growth stage and fatty acid accumulation stage.T.cutaneum lipid was mainly comprised of oleic acid(C18:1),palmitic acid(C16:0),palmitoleic acid(C16:1).The fatty acid composition was as follows:oleic acid(C18:1)64.80%,palmitic acid(C16:0)19.90%,palmitoleic acid(C16:1)10.94%,stearic acid(C18:0)3.72% and linoleic acid(C1... |
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| Keywords: | microorganism cell lipid content fatty acid composition gas chromatography-mass spectrometry(GC-MS) |
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