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| 褐藻寡糖检测方法的建立 | |
| 引用本文： | 聂莹,张文靖,石波,韭泽悟,苏东海,程永强.褐藻寡糖检测方法的建立[J].中国食物与营养,2012,18(8):29-33. |
| 作者姓名： | 聂莹 张文靖 石波 韭泽悟 苏东海 程永强 |
| 作者单位： | 1. 中国农业大学,北京,100083 2. 中国农业科学院饲料研究所,北京,100081 3. 日本国际农林水产业研究中心,日本筑波305-8686 4. 北京电子科技职业学院,北京,100029 |
| 基金项目： | 国家自然科学基金项目（项目编号：30972286和31171739）;教育部新世纪优秀人才支持计划项目（项目编号：NETC-10-0776）;教育部2011年度基本科研业务费支持项目;北京市优秀人才培养资助计划（项目编号：2011D009007000001）;北京市属高等学校人才强教计划资助项目. |
| 摘 要： | 通过不同的检测方式建立褐藻寡糖的快速定性检测方法,为褐藻寡糖(AOS)的制备提供快速而准确的检测依据。结果表明,相同的褐藻寡糖样品通过薄板层析法(TLC)、荧光辅助糖电泳法(FACE)以及高效液相色谱法(HPLC)均可以得到较为理想的检测结果。优化后的3种方法的检测条件依次为:TLC法:正丁醇∶甲酸∶水=4∶5∶1,上行展开两次;FACE法:酶解及酸解的褐藻寡糖经过8-氨基萘-1,3,6-三磺酸(ANTS)胺化还原衍生16h,适宜的聚丙烯酰胺凝胶浓度分别为25%、35%,恒压400V电泳1h后,1 000V电泳4h;HPLC法:色谱柱:TSK-GEL DEAE-2SW 4.6mm×250mm;流动相:0—0.25MNaCl在60min内梯度洗脱;流速:0.8 mL/min、波长:230nm。 |
| 关 键 词： | 褐藻寡糖 荧光辅助糖电泳法 高效液相色谱法 薄板层析法 |
Establishment of Dectection Methods for Alginate Oligosaccarides |
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| NIE Ying,ZHANG Wen-jing,SHI Bo,Nirasawa Satoru,SU Dong-hai,CHENG Yong-qiang.Establishment of Dectection Methods for Alginate Oligosaccarides[J].Food and Nutrition in China,2012,18(8):29-33. | |
| Authors: | NIE Ying ZHANG Wen-jing SHI Bo Nirasawa Satoru SU Dong-hai CHENG Yong-qiang |
| Affiliation: | 1 College of Food Sciense and Nutritional Engineering, China Agricultural University, Beijing 100083, China ; 2 Feed Research Institute, Chinese Academy of Agriculture Sciences, Beijing 100081, China; Japan International Research Center for Agricultural Scierwes, Tsukuba 305-8686, Japan ; 4 Beifing Polytechnic Institute, Beijing 100029, China) |
| Abstract: | The research established the quick detection methods for Alginate Oligosaccharides （AOS） by applying different kinds of analytical equipments. The results showed that FACE, HPLC and TLC could offer ideal methods for the analysis of AOS. The proper analytical conditions for FACE, HPLC and TLC were that TLC： 1-buthanol-formie acid-water = 4：5：1 （V/V/V）, chromatographed for twice; FACE ： the ANTS labeled AOS prepared by both enzymatic actions and hydrolysis ： on a 25% acrylamide gel and 35% acrylamide gel perspectively, was both electrophoresed at 400V for 1 h, followed by 1 000V for 4h ; HPLC ： the column was TSK-GEL DEAE-2SW 4. 6mm x 250mm, mobile phase was deionized 0-43. 25 M NaC1 with a linear gradien in 60min, flow rate was 0. 8mL/min, and h was 230nm. |
| Keywords: | Alginate Oligosaccharides FACE HPLC TLC |
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