| 高效液相色谱法测定圣女果中维生素C的含量 |
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| 引用本文： | 刘嘉祺,孙仁国.高效液相色谱法测定圣女果中维生素C的含量[J].中国食物与营养,2012,18(7):69-70. |
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| 作者姓名： | 刘嘉祺 孙仁国 |
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| 作者单位： | 1. 牡丹江医学院,黑龙江牡丹江,157011
2. 牡丹江市质检中心,黑龙江牡丹江,157009 |
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| 摘 要： | 建立了一种高效液相色谱测定圣女果中维生素C含量的方法。色谱中采用Hypersil-C18色谱柱，0．1％草酸作流动相，流速1．0mL／min，紫外检测器，检测波长254nm。维生素C在20-100mg／L内有良好的线性关系，线性回归方程为y=51．59x-55．12，相关系数R2=0．9998；5，回收率为97．88％-101．52％，相对标准偏差等于0．15％。所用测定方法简便、快速，能准确测定圣女果中维生素C的含量。
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| 关 键 词： | 圣女果 高效液相色谱 维生素C |
Detection of Content of Vitamin C in Cherry Tomatoes by High Performance Liquid Chromatography |
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| LIU Jia-qi,SUN Ren-guo.Detection of Content of Vitamin C in Cherry Tomatoes by High Performance Liquid Chromatography[J].Food and Nutrition in China,2012,18(7):69-70. |
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| Authors: | LIU Jia-qi SUN Ren-guo |
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| Affiliation: | (IMudanjiang Medical University, Mudanjiang 157011, China; 2Mudanjiang City Quality Inspection and Testing Center, Mudanjiang 157009, China) |
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| Abstract: | A practical method was established by HPLC to detect the content of Vitamin C in cherry tomatoes. A Hypersil-C18 column was used and the mobile phase was 0.1% oxalic acid. The detector was UV and the wave length was 254 nm. The flow rate was 1.0mL/ min in the HPLC system. The standard working curve of Vitamin C had a good linear correlation in the range from 20 to 100 mg/L, the regression equation was y=31.59x-53.12, and coefficient correlation（ R2） was 0.99983. The recoveries were 97.88%-101.52% and RSD was 0.15 %. The method was simple and fast, and could determine the content of Vitamin C in the cherry tomatoes accurately. |
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| Keywords: | cherry tomato HPLC Vitamin C |
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