纤维素酶—微波辅助提取姜辣素的研究

以生姜粉为原料,首先用纤维素酶处理破坏生姜细胞结构,然后采用微波间歇辅助乙醇溶剂提取。探讨原料固液比,酶解温度,酶添加量,酶处理时间和微波处理时间在不同的水平下对姜辣素得率的影响,初步筛选提取条件,通过单因素试验和L9(34)正交试验,确定纤维素酶—微波辅助提取姜辣素的最佳工艺为固液比(g/mL)为2.0∶200,酶解温度50℃,酶添加量4%,酶处理时间100min,微波辐射时间为80s。粗提物中姜辣素含量为6.17%。

The Study of Gingerol Extraction by Cellulase and Microwave Assistant

Gingerol was extracted from ginger powder,then use cellulase to broken the ginger cell structure.Discuss the solid-to-liquid ratio,enzymolysis temperature,additional amount of Enzyme,the enzyme process-time and microwave process-time which have the affection on the rate of gingerol.The optimum technology was obtained by single-factor test and L9(34) orthogonal test.The results showed that the optimum solid-to-liquid ratio is 2.0∶200,hydrolysis temperature is 50 ℃,enzyme dose is 4%,treating time is 100 mins,microwave irradiation time is 80 s.Content of gingerol obtained by this technology can reach up to 6.17% in crude extract.