| 有关剪切对紫苏植物细胞存活率的影响的定量分析 |
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| 引用本文： | 钟建江 吉田敏臣. 有关剪切对紫苏植物细胞存活率的影响的定量分析[J]. 化学反应工程与工艺, 1994, 10(4): 392-396 |
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| 作者姓名： | 钟建江 吉田敏臣 |
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| 作者单位： | 华东理工大学生化工程研究所，大阪大学工学部生物工学国际交流中心 |
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| 摘 要： | 用回转筒反应器针对剪切力紫苏植物细胞的存活率的影响作了定量研究。结果表明剪切力剪切明间明显细胞存活率，细胞的生长期和细胞浓度对处于剪切状态下细胞存活率有较大影响。处于静止细胞比处于指数生长期的细胞对剪切更敏感。
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| 关 键 词： | 剪切力 紫苏 植物细胞 回转圆筒反应器 存活率 |
A QUANTITATVE ANALYSLS OF EFFECT OF SHEAR STRESS ON SUSPENDED CELLS OF PERILL FRUTESCENS |
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| Zhong Jianjiang, Yu Juntang. A QUANTITATVE ANALYSLS OF EFFECT OF SHEAR STRESS ON SUSPENDED CELLS OF PERILL FRUTESCENS[J]. Chemical Reaction Engineering and Technology, 1994, 10(4): 392-396 |
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| Authors: | Zhong Jianjiang Yu Juntang |
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| Abstract: | Effects of shear stress on suspend cells of Perilla frutescens were quantitatively investi-gated in a well-defined shear field by use of a rotating drum reactor. Both shear stress and shearing time strongly affected cell viability. Growth state and concentration of cells also showed significant effects on the cell viability,i.e.,the cells at stationary phase were more susceptible to shear force than those at exponential phase,and the cell viability was higher at a higher cell concentration. Moreover, the results indicate that an addition of pluronic F-68 showed to be protective towards the suspended cells of P. frutescens from shear damage in the reactor. |
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| Keywords: | Shear stress Perilla frutescens Plant cell culture Rotathg drum reactor Cell viability Pluronic F-68. |
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