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槟榔嫩果中儿茶素的液相色谱质谱联用测定
引用本文:王明月,罗金辉,赵振东. 槟榔嫩果中儿茶素的液相色谱质谱联用测定[J]. 食品科学, 2010, 31(6): 196-198. DOI: 10.7506/spkx1002-6630-201006045
作者姓名:王明月  罗金辉  赵振东
作者单位:1.中国热带农业科学院热带生物技术研究所 2.中国热带农业科学院分析测试中心 3.海南大学分析测试中心
基金项目:2008年中央级公益性科研院所基本科研业务费项目(ITBBKF083)
摘    要:采用甲醇超声提取槟榔嫩果中的儿茶素,先用石油醚除去杂质,再用乙酸乙酯萃取,提取物经抽真空浓缩,高效液相色谱- 质谱联用法测定。结果表明:槟榔嫩果中儿茶素类组分为(+)- 儿茶素和表没食子儿茶素没食子酸酯。

关 键 词:高效液相色谱-质谱法  槟榔嫩果  儿茶素  
收稿时间:2009-07-01

HPLC-MS Determination of Catechins in Tender Areca Nut
WANG Ming-yue,,LUO Jin-hui,ZHAO Zhen-dong. HPLC-MS Determination of Catechins in Tender Areca Nut[J]. Food Science, 2010, 31(6): 196-198. DOI: 10.7506/spkx1002-6630-201006045
Authors:WANG Ming-yue    LUO Jin-hui  ZHAO Zhen-dong
Affiliation:1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101,China ;2. Analysis and Testing Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;3. Analysis and Testing Center, Hainan University, Haikou 570228, China
Abstract:An Agilent HPLC-MS system was used to determine catechins in tender areca nut.Sample preparation was achieved through methanol extraction with the assistance of ultrasonic,petroleum ether extraction for the removal of impurities,ethyl acetate extraction and vacuum concentration.Results showed that catechins in tender areca nut mainly comprised(+)-catechin and epigallocatechin gallate.
Keywords:high-performance liquid chromatography-mass spectrometry(HPLC-MS)  tender areca nut  catechins  
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