马铃薯蛋白与蛋清蛋白混合凝胶特性分析

以马铃薯蛋白(potato protein,PP)和蛋清蛋白(egg white protein,EWP)为原料制备热诱导混合凝胶,分析不同比例PP-EWP混合凝胶的质构、蛋白质二级结构、分子间作用力、游离巯基及流变性质的变化规律.结果 表明,随着PP含量的增加,混合凝胶的保水性由(73.5±0.71)％升高至(97....

Gelling Properties of Potato Protein-Egg White Mixtures

In this study, heat-induced gels were made from mixtures of potato protein (PP) and egg white protein (EWP) at different ratios, and their texture properties, protein secondary structures, intermolecular forces, free sulfhydryl group contents and rheological properties were investigated. The results showed that with increasing level of PP, the water-holding capacity of mixed gels increased from (73.5 ± 0.71)% to (97.5 ± 0.71)% (P < 0.05) and the roughness gradually decreased. With increasing level of EWP, the hardness increased from (460.5 ± 4.4) g to (1 614.9 ± 126.4) g (P < 0.05). The two proteins complemented each other in water-holding capacity and hardness. When the ratio of PP to EWP approached 1, the secondary structure of mixed gels was transformed from β-sheet and α-helical to random coil, and the hydrogen bonds were gradually weakened. Hydrophobic interactions and free sulfhydryl contents increased with increasing level of EWP. The mixed gels with PP-EWP ratio of 1:0, 9:1 and 0:1 possessed higher elastic modulus. The gels strength was significantly positively correlated with hydrophobic interactions and free sulfhydryl group content (P < 0.01), and negatively correlated with water-holding capacity (P < 0.01), but had no significant correlation with storage modulus (P > 0.05).