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DNA‐Scaffolded Multivalent Ligands to Modulate Cell Function |
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| Authors: | Prof. Dr. Zhiqing Zhang Dr. Mark A. Eckert Dr. M. Monsur Ali Linan Liu Dr. Dong‐Ku Kang Elizabeth Chang Dr. Egest J. Pone Prof. Dr. Leonard S. Sender Prof. Dr. David A. Fruman Prof. Dr. Weian Zhao |
| Affiliation: | 1. State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, 66 Changjiang (West) Rd, Huangdao, Qingdao, 266580 (P. R. China);2. Department of Pharmaceutical Sciences, Department of Biomedical Engineering, Sue and Bill Gross Stem Cell Research Center and Chao Family Comprehensive Cancer Center, and Edwards Lifesciences Center for Advanced Cardiovascular Technology, University of California, Irvine, 845 Health Sciences Road, Irvine, CA 92697 (USA) http://faculty.sites.uci.edu/zhaolab/;3. Department of Medicine, University of California, Irvine, Hyundai Cancer Institute, CHOC Children's Hospital, 1201 W. La Veta Avenue, Orange, CA 92697 (USA);4. Department of Molecular Biology & Biochemistry, University of California, 3242 McGaugh Hall, Irvine, CA 92697 (USA) |
| Abstract: | We report a simple, versatile, multivalent ligand system that is capable of specifically and efficiently modulating cell‐surface receptor clustering and function. The multivalent ligand is made of a polymeric DNA scaffold decorated with biorecognition ligands (i.e., antibodies) to interrogate and modulate cell receptor signaling and function. Using CD20 clustering‐mediated apoptosis in B‐cell cancer cells as a model system, we demonstrated that our multivalent ligand is significantly more effective at inducing apoptosis of target cancer cells than its monovalent counterpart. This multivalent DNA material approach represents a new chemical biology tool to interrogate cell receptor signaling and functions and to potentially manipulate such functions for the development of therapeutics. |
| Keywords: | cancer CD20 DNA nanotechnology multivalency rolling circle amplification |