Biosynthesis of Hygrocins,Antitumor Naphthoquinone Ansamycins Produced by Streptomyces sp. LZ35 |
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| Authors: | Dr. Shanren Li Dr. Haoxin Wang Dr. Yaoyao Li Jingjing Deng Dr. Chunhua Lu Yan Shen Prof. Dr. Yuemao Shen |
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| Affiliation: | 1. State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, No. 27 Shanda South Road, Jinan 250100 (P. R. China);2. School of Life Science, Xiamen University, No. 422 South Siming Road, Xiamen 361005 (P. R. China);3. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, No. 44 Wenhua West Road, Jinan, Shandong 250012 (P. R. China) |
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| Abstract: | Hygrocins are naphthoquinone ansamycins with significant antitumor activities. Here, we report the identification and characterization of the hygrocin biosynthetic gene cluster (hgc) in Streptomyces sp. LZ35. A biosynthetic pathway is proposed based on bioinformatics analysis of the hgc genes and intermediates accumulated in selected gene disruption mutants. One of the steps during the biosynthesis of hygrocins is a Baeyer–Villiger oxidation between C5 and C6, catalyzed by luciferase‐like monooxygenase homologue Hgc3. Hgc3 represents the founding member of a previously uncharacterized family of enzymes acting as Baeyer–Villiger monooxygenases. |
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| Keywords: | antibiotic Baeyer– Villiger monooxygenases biosynthesis hygrocins luciferase‐like monooxygenases |
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